Niranjan V. Kulkarni
CV

Biomedical optics · Optical coherence tomography · Neuroimaging

Niranjan Vinay Kulkarni

Research Technician, Adie Lab, Cornell University · Ithaca, NY, USA

I study how optical coherence tomography signals relate to tissue structure in the brain, combining quantitative signal analysis, neuroimaging workflows, and biological validation.

Research

Current research

Biomedical optics and photonics, with a focus on optical coherence tomography, light scattering in biological tissue, and structure-function relationships in the brain.

1300 nm OCT

Quantitative OCT of brain tissue

Analyzing depth-dependent OCT A-line profiles to understand how scattering-driven contrast varies across brain regions and what structural features the signal represents.

  • Conducts ex vivo brain imaging using 1300 nm OCT.
  • Studies region-wise structural differences, including white matter versus gray matter.
  • Implements dispersion compensation and Fourier-domain reconstruction to improve depth-resolved signal consistency.
  • Investigates relationships between scattering behavior and underlying tissue structure.
1300 nm sourceReference mirrorDetectorSample armex vivo brain tissuebeamsplitter

Illustrative swept-source OCT sample arm at 1300 nm, imaging ex vivo brain tissue.

Where it goes

Relate quantitative OCT signal behavior to underlying tissue structure and regional differences in the brain.

In vivo / ex vivo workflow

In vivo neuroimaging and biological validation

Builds experimental workflows that connect optical imaging with small-animal disease models and histological ground truth.

  • Established and leads the lab's small-animal neuroimaging model workflow.
  • Rewrote and executes IACUC protocols for 5xFAD Alzheimer's and GL261 glioblastoma models.
  • Performed and optimized more than 20 cranial-window implantation surgeries for longitudinal in vivo imaging.
  • Established cryosectioning, Nissl staining, and H&E staining workflows for OCT–histology comparison.
  • Contributes biological validation and experimental-pipeline design to ultra-deep OCT development.
Cranial-windowimplantationLongitudinalin vivo OCTCryosectioning& stainingHistologycomparison

Illustrative cranial-window imaging workflow: surgery, longitudinal OCT, and histological validation.

Where it goes

Ground optical signal behavior in disease-model biology and histological ground truth, not signal analysis alone.

Featured system

Columnar brain biopsy device

Outer cannulaprotects healthy tissueInner cannulacollects columnar sampleColumnar tissue core

Fig. 1 — Concept schematic: dual-cannula columnar biopsy device (illustrative, not to scale).

Design Engineer — Touchdown MedTech, Cornell University · August 2024 – May 2025

A novel device for columnar brain biopsy

Designed and iterated a novel device for columnar brain biopsy in collaboration with Cornell Engineering and Weill Cornell Medicine. The device is a dual-cannula concept designed to collect larger, intact columnar brain-tissue samples while protecting healthy tissue.

Cornell's public showcase reports successful testing and validation indicating increased sample volume and integrity over conventional biopsy approaches.

Prototypes built50+ (Fusion 360, iterative/AGILE)
Documentation standardFDA CFR 820
Regulatory pathway exploredFDA 510(k)
RecognitionCornell M.Eng. Design Award for Engineering Excellence, 2025
Review panel10+ industry professionals and experts

Exhibited as “A Novel Device for Conducting Columnar Brain Biopsies” at the Cornell Engineering Design Expo, 2025, with Andrew Yang, Lilly Goodwin, Anna Gwozdz, Anna Olivia Humiston, Niranjan Vinay Kulkarni.

Cornell Engineering project source

Publications

Selected publications

Experience

Research and professional experience

Bench capabilities

Skills

Factual capability groups, not proficiency ratings.

Optical imaging

  • Optical Coherence Tomography (OCT)
  • Optical Coherence Microscopy (OCM)
  • Confocal microscopy

Data analysis

  • MATLAB
  • Python
  • ImageJ

Signal processing

  • Fourier-domain reconstruction
  • Dispersion compensation
  • Phase stability
  • Depth-dependent signal analysis

Instrumentation and design

  • Interferometric alignment
  • Optical assembly
  • 3D CAD
  • Autodesk Fusion 360
  • SolidWorks

Materials characterization

  • XRD
  • SEM
  • UV-Vis
  • DLS / optical-property analysis

Software

  • LabVIEW
  • Autodesk Fusion 360
  • OriginPro

Biological methods

  • Cranial-window implantation
  • Ex vivo brain extraction
  • Tissue fixation
  • Cryosectioning
  • Nissl staining
  • H&E staining
  • GL261 tumor implantation
  • In vitro neuronal culture

Education

Education

August 2024 – May 2025

M.Eng. Biomedical Engineering

Cornell University

  • Biomedical Optics
  • Neurotechnology
  • Neurophysiology

August 2020 – May 2024

B.Tech. Bioengineering

Vellore Institute of Technology

  • Computational Biology and Analytics
  • Biosignals and Image Processing
  • Cancer Biology
  • Python Programming
  • Immune Engineering

Record

Teaching, mentorship, and leadership

Teaching & mentorship

  • Mentors five undergraduate researchers

    Current

    Adie Lab, Cornell University

    • Experimental design, imaging workflows, and data interpretation.
  • Project Mentor

    March 2024 – May 2024

    SMART Lab, IIT Indore

  • Laboratory Teaching Assistant

    September 2022 – December 2022

    Tissue Engineering Lab, VIT Bhopal

Leadership & activities

  • Co-Founder and Secretary, The Bioengineering Club

    2021 – 2022
    • Helped establish a student body of 100+ students connecting students with professionals in biomedical fields.
    • Organized scientific coffee-table forums and the RABVIT '23 bioengineering symposium on contemporary healthcare issues in India.
  • MIT GrandHack '25

    2025
    • Participant and finalist, Heart Health track.
  • Student Member, Unnat Bharat Abhiyaan

    2022
    • Volunteered in rural areas of central India on a survey project.
  • Volunteer, Mauli Foundation

    2021 – 2023
    • Led outreach for farmers on scientifically validated agricultural practices and soil-treatment methods.
    • Conducted workshops on nano-formulations for improved seed germination.

Selected project

  • Bio-inspired Global Positioning Systems based Joint Precision Air Drop System

    October 2022 – April 2023

    VIT Bhopal

    • Conducted a statistical survey across 20+ healthcare institutions and collaborated with stakeholders to understand healthcare-access problems in rural India.
    • Designed and modeled 8+ medical-kit payloads based on survey findings.
    • Collaborated with an interdisciplinary team spanning four majors.